Grant‐in‐Aid for Scientific Research on Innovative Areas
(Research in a proposed research area)

Novel measurement techniques forvisualizing 'live' protein molecules at work

Members

Management Group

Group Leader :Daisuke Kohda

(Kyushu University)

Group Member

Toshio Ando (Kanazawa University)
Masahiro Shirakawa (Kyoto University)
Noritaka Nishida (The University of Tokyo)
Yuji Sugita (Riken)
Tomoya Tsukazaki
(Nara Institute of Science and Technology)

Collaborator

Masatsune Kainosho
(Tokyo Metropolitan University)
Tama Florence (Nagoya University)
Hideo Akutsu (Yokohama City University)
Ichio Shimada (The University of Tokyo)
Yoshimumi Nishimura
(Yokohama City University)

Adviser

Toshiya Endo (Kyoto Sangyo University)
Haruki Nakamura (Osaka University)
Kosuke Morikawa (Kyoto University)

Research Projects(2017〜2018)

Group
A01

Innovative measurement techniques for
the dynamic analyses of transient protein complexes

Toshio Ando(Kanazawa University)
"Development of advanced high-speed AFM and analysis of proteins' action mechanism"
Daisuke Kohda(Kyushu University)
"Intentional creation of crystal contact-free space for analyzing large amplitude
motions in protein crystals"
Tomohide Saio(Hokkaido University)
Masaki Okumura(Tohoku University)
Youhei Miyanoiri(Osaka University)
Takashi Nagata(Kyoto University)
Tetsunari Kimura(Kobe Univeristy)
Michihiro Suga(Okayama University)
Hiroaki Yokota(The Graduate School for the Creation of New Photonics Industries)
Keio Hirose(AIST)
Akihiko Nakamura(Institute for Molecular Science)
Group
A02

Innovative measurement techniques for
the dynamic analyses of protein molecules under
physiological conditions

Noritaka Nishida(The University of Tokyo)
"Real-time observation of biological events inside living cells by new in-cell NMR techniques"
Masahiro Shirakawa(Kyoto University)
"ODMR and in-cell NMR techniques for analyzing protein dynamics and
protein-protein interactions in situ (tentative)"
Takakazu Nakabayashi(Tohoku University)
Masayuki Murata(The University of Tokyo)
Rikiya Watanabe(The University of Tokyo)
Satosi Abe(Tokyo Institute of Technology)
Chojiro Kojima(Yokohama National University)
Richard Wong(Kanazawa University)
Hiroyuki Mori(Kyoto Univeristy)
Masayoshi Nishiyama(Kyoto University)
Yutaka Ito(Tokyo Metropolitan University)
Takahiro Ide(Riken)
Group
A03

Validation and application of the new innovative
measurement techniques

Tomoya Tsukazaki(Nara Institute of Science and Technology)
"Visualization of Sec Translocon Machinery"
Yuji Sugita(Riken)
"Molecular dynamics simulations of protein complexes based on multi-resolution models"
Ayako Furukawa(Yokohama City University)
Hirofumi Shimizu(University of Fukui)
Asako Furukohri(Nara Institute of Science and Technology)
Masaki Mishima(Tokyo Metropolitan University)
Hirokazu Yagi(Nagoya City University)
Masayoshi Nakasako(Keio University)
Saeko Yanaka(Institute for Molecular Science)
Yuko Fujioka(Microbial Chemistry Research Foundation)
Hiroshi Sugimoto(Riken)
Hideki Shigematsu(Riken)

Research Projects (2015〜2016)

Group
A01

Innovative measurement techniques for
the dynamic analyses of transient protein complexes

Daisuke Kohda (Kyushu University) Lab. ホームページ
"Intentional creation of crystal contact-free space for analyzing large amplitude
motions in protein crystals"
Toshio Ando (Kanazawa University) Lab. ホームページ
"Development of advanced high-speed AFM and analysis of proteins' action mechanism"
Tomohide Saio (Hokkaido University) Lab. ホームページ
Kiyoto Kamagata (Tohoku University) Lab. ホームページ
Masaki Okumura (Tohoku University) Lab. ホームページ
Junichiro Yajima (The University of Tokyo) Lab. ホームページ
Takashi Nagata (Kyoto University) Lab. ホームページ
Misao Mizuno (Osaka University) Lab. ホームページ
Michihiro Suga (Okayama University) Lab. ホームページ
Takuo Yasunaga (Kyushu Institute of Technology) Lab. ホームページ
Naoya Shibayama (Jichi Medical University) Lab. ホームページ
Hiroaki Yokota (The Graduate School for the Creation of
New Photonics Industries) Lab. ホームページ
Yuko Fujioka (Microbial Chemistry Research Foundation) Lab. ホームページ
Mitsuhiro Suga (The University of Tokyo) Lab. ホームページ
Group
A02

Innovative measurement techniques for
the dynamic analyses of protein molecules under
physiological conditions

Masahiro Shirakawa (Kyoto University) Lab. ホームページ
"ODMR and in-cell NMR techniques for analyzing protein dynamics and
protein-protein interactions in situ (tentative)"
Noritaka Nishida (The University of Tokyo) Lab. ホームページ
Masatsune Kainosho (Tokyo Metropolitan University) Lab. ホームページ
"Real-time observation of biological events inside living cells by new in-cell NMR techniques"
Masayuki Murata (The University of Tokyo) Lab. ホームページ
Rikiya Watanabe (The University of Tokyo) Lab. ホームページ
Tai Kiuchi (Kyoto University) Lab. ホームページ
Tochio Hidehito (Kyoto University) Lab. ホームページ
Itaru Hamachi (Kyoto University) Lab. ホームページ
Yutaka Ito (Tokyo Metropolitan University) Lab. ホームページ
Kenji Okamoto (Riken) Lab. ホームページ
Group
A03

Validation and application of the new innovative
measurement techniques

Yuji Sugita (Riken) Lab. ホームページ
Tama Florence (Nagoya University) Lab. ホームページ
"Molecular dynamics simulations of protein complexes based on multi-resolution models"
Tomoya Tsukazaki (Nara Institute of Science and Technology) Lab. ホームページ
"Visualization of Sec Translocon Machinery"
Takashi Okuno (Yamagata University) Lab. ホームページ
Hirofumi Shimizu (University of Fukui) Lab. ホームページ
Yasuhisa Kimura (Kyoto University) Lab. ホームページ
Toru Minamino (Osaka University) Lab. ホームページ
Shiro Suetsugu (Nara Institute of Science and Technology) Lab. ホームページ
Masayoshi Nakasako (Keio University) Lab. ホームページ
Miho Katsuki (Fukuoka University) Lab. ホームページ
Ayako Furukawa (Yokohama City University) Lab. ホームページ
Hiroshi Sugimoto (Riken) Lab. ホームページ
Hideki Shigematsu (Riken) Lab. ホームページ

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