九州大学 生体防御医学研究室 構造生物学分野 TRUE COMBINATION OF XRAY,NWR AND EM FOR STURUCTURAL BIOLOGY

Laboratory Staff

Daisuke Kohda, Ph.D.

Daisuke Kohda

Daisuke Kohda

Professor / Division of Structural Biology
TEL +81 (0)92 642 6968
FAX +81 (0)92 642 6833
MAIL kohda[at]bioreg.kyushu-u.ac.jp

Feb 1959
birth (TOKYO)
Apr 1974~
Mar 1977
Shonan High School (KANAGAWA)
Apr 1977~
Mar 1981
the University of Tokyo, Biochemistry
Apr 1981~
Mar 1983
the University of Tokyo, Biochemistry(BS)
Apr 1983~
Mar 1986
the University of Tokyo, Biochemistry (Ph.D.)
Apr 1986~
Dec 1995
Research scientist, the Tokyo Metropolitan Institute of Medical Science (TOKYO)
(Jul 1994-Jul 1995: visiting scientist, c/o Prof. Iain D. Campbell's laboratory (Oxford University), with a research fellowship from Japan Society for the Promotion of Science)
Jan 1996~
Mar 2000
Senior research scientist, Biomolecular Engineering Research Institute (OSAKA)
Apr 2000~
Mar 2002
Principal research scientist, Biomolecular Engineering Research Institute (OSAKA)
Apr 2002~
Professor, Medical Institute of Bioregulation, Kyushu University (FUKUOKA)
Apr 2008~
Mar 2010
Vice-President, Medical Institute of Bioregulation, Kyushu University (FUKUOKA)
Sep 2014~
Distinguished Professor, Kyushu University (FUKUOKA)
We are using X-ray crystallography, nuclear magnetic resonance (NMR), and electron microscopy. Although structure determination is the essential part of our approach, we emphasize that structures themselves are not a goal, but a starting point of studies. Protein structures inspire the design of new biochemical experiments, and the feedbacks produce new targets for structural biology in the future. The tight coupling of structure determination and biological experiments is essential for successful structural studies.
Read More

1. Solution Structure of the SH3 Domain of Phospholipase Cγ
D. Kohda, H. Hatanaka, M. Odaka, V. Mandiyan, A. Ullrich, J. Schlessinger, and F. Inagaki
Cell 72, 953-960 (1993).
PubMed: 0007681365

2. Solution Structure of the Link Module: A Hyaluronan-Binding Domain Involved in Extracellular Matrix Stability and Cell Migration
D. Kohda. C. J. Morton, A. A. Parkar, H. Hatanaka, F. M. Inagaki, I. D. Campbell, and A. J. Day
Cell, 86, 767-775 (1996).
PubMed: 0008797823

3. Disulfide Bond Structure of Human Epidermal Growth Factor Receptor
Y. Abe, M. Odaka, F. Inagaki, I. Lax, J. Schlessinger, and D. Kohda
J. Biol. Chem. 273, 11150-11157 (1998).
PubMed: 0009556602

4. Structural Basis of Presequence Recognition by the Mitochondrial Protein Import Receptor Tom20
Y. Abe, Toshihiro S., T. Muto, K. Mihara, H. Torii, S. Nishikawa, T. Endo, and D. Kohda
Cell 100, 551-560 (2000).
PubMed: 0010721992

5. Solution Structure of the PX domain, a Target of the SH3 domain
H. Hiroaki, T. Ago, T. Ito, H. Sumimoto, and D. Kohda
Nature Struct. Biol. 8, 526-530 (2001).
PubMed: 0011373621

6. Tom20 recognizes mitochondrial presequences through dynamic equilibrium among multiple bound states.
Saitoh T, Igura M, Obita T, Ose T, Kojima R, Maenaka K, Endo T, Kohda D
Embo J 26, 4777-4787 (2007).
PubMed: 0017948058
This paper was selected as "Must Read " by Faculty of 1000 Biology (http://www.f1000biology.com/)

7. Structure-guided identification of a new catalytic motif of oligosaccharyltransferase.
Igura M, Maita N, Kamishikiryo J, Yamada M, Obita T, Maenaka K, Kohda D
Embo J 27, 234-243 (2008).
PubMed: 0018046457

8. Crystal structures of an archaeal oligosaccharyltransferase provide insights into the catalytic cycle of N-linked protein glycosylation
Matsumoto S, Shimada A, Nyirenda J, Igura M, Kawano Y, Kohda D
Proc Natl Acad Sci USA 110, 17868-17873 (2013).
PubMed: 24127570

9. Rational design of crystal contact-free space in protein crystals for analyzing spatial distribution of motions within protein molecules
Matsuoka R, Shimada A, Komuro Y, Sugita Y, Kohda D
Protein Sci 25, 754–768 (2016).
PubMed: 26694222

10. Comparative Analysis of Archaeal Lipid-linked Oligosaccharides That Serve as Oligosaccharide Donors for Asn Glycosylation
Taguchi Y, Fujinami D, Kohda D
J Biol Chem 291, 11042–11054 (2016).
PubMed: 27997792

Reviews

1. Functions of outer membrane receptors in mitochondrial protein import
Endo T, Kohda D
Biochim Biophys Acta 1592, 3-14 (2002).
PubMed: 12191763

2. Structural Biology of Oligosaccharyltransferases (Pages 437-445)
Matsumoto S, Nyirenda J, Kohda D
in Glycoscience: Biology and Medicine
Publisher: Springer Japan
Editors: Taniguchi, N., Endo, T., Hart, G.W., Seeberger, P.H., Wong, C.-H.
Year of publication: 2014
print ISBN: 978-4-431-54840-9, e-book ISBN: 978-4-431-54841-6

3. Chapter 21: Protein ligand interactions studied by NMR
Hiroaki H, Kohda D
in Experimental approaches of NMR spectroscopy -Methodology and application to life science and materials science-
Publisher: Springer Singapore
Editor: The Nuclear Magnetic Resonance Society of Japan
Year of publication: 2017
print ISBN: 978-981-10-5965-0, e-book ISBN: 978-981-10-5966-7

Full list of publications and invited oral talks
(1984 - Mar 2017)

Labo members
April, 2017

OB/OG

Group Photos

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